other:
Western Blot:Article Title: Hepatitis B surface antigen is upregulated by HIV Tat in an HIV–hepatitis B virus co-infection model system
Article Snippet: .. Commercial primary antibodies used for Western blotting, immunofluorescence and flow cytometry were rabbit anti-HBV preS2 (PA5-22820; Invitrogen), rabbit anti-HBsAg (NB100-62652; Novas Biologicals, Littleton, CO), mouse anti-FLAG M2 (F1804; Sigma-Aldrich), rabbit anti-GFP (ab290; Abcam, Cambridge, UK), rabbit anti-GAPDH (2118; Cell Signaling Technology, Danvers, MA), rabbit anti-RNA polymerase II CTD, phospho S2 (ab193467; Abcam), and SureLight APC mouse anti-DDDDK tag (ab72569; Abcam). .. Anti-mouse IgG-HRP and anti-rabbit IgG-HRP or Alexa Fluor 488 and 568 (A11001, A11008, A11011, and A11031; Invitrogen) were used as secondary antibodies for Western blotting or immunofluorescence.
Article Title: The endoplasmic reticulum degradation-enhancing α-mannosidase-like protein 3 attenuates the unfolded protein response and has pro-survival and pro-viral roles in hepatoma cells and hepatocellular carcinoma patients
Article Snippet: Samples consisting of 50 tumor tissue (T), 50 normal adjacent to tumor tissue (NAT) and 10 normal (N) liver tissues were collected at the time of surgery in RNA stabilization solution (Sigma-Aldrich) and stored at −80 °C for further analysis. .. Primary antibodies used for western blot analyses include mouse anti-EDEM3 (E0409, Sigma-Aldrich, 1:500), rabbit anti-EDEM1 (E8406, Sigma-Aldrich, 1:1000), rabbit anti-EDEM2 (JD-32) (sc - 130460, Santa Cruz Biotechnology, 1:250), rabbit anti-HBsAg (NB100-62652, Novus Biologicals, Littleton, CO, USA, 1:1000), rabbit anti-phospho-IRE1 alpha (Ser724) (PA1 - 16927, Invitrogen, 1:1000), rabbit anti-IRE1α (14C10, Cell Signaling Technology, Danvers, MA, USA, 1:1000), mouse anti - ATF6 (70B1413.1, Novus Biologicals, 1:1000), anti-glucose-regulated protein 94 (GRP94; H-10, Santa Cruz Biotechnology, 1:500), rabbit anti - phospho - PERK-T980 (16F8, Cell Signaling Technology, 1:1000), rabbit anti-PERK (C33E10, Cell Signaling Technology, 1:1000), rabbit anti - phospho-alpha subunit of eukaryotic initiation factor 2, S51(p - eIF2α; 9721S, Cell Signaling Technology, 1:1000), rabbit anti-eIF2α (9722S, Cell Signaling Technology, 1:1000), mouse anti-binding immunoglobulin protein (GRP78/BiP; A-10; sc-376768, Santa Cruz Biotechnology, 1:200), rabbit anti-suppressor of Lin - 12 - like (Sel1L; ab78298, Cell Signaling Technology, 1:500), rabbit anti-HMG-CoA reductase degradation protein 1, also known as Synoviolin (Hrd1; 14773, Cell Signaling Technology, 1:1000), rabbit anti-ER lectin 1 (XTP3-B/ERLEC1; ab181166, Abcam, Cambridge, UK, 1:500), rabbit anti-osteosarcoma amplified - 9 (OS-9; ab19853, Abcam, 1:500), rabbit anti-Ras homolog enriched in the brain (Rheb; E1G1R, Cell Signaling Technology, 1:1000), goat anti-apolipoprotein E (ApoE; AB947, Sigma-Aldrich, 1:5000), rabbit anti-microtubule-associated protein light chain 3A/B (LC3A/B; D3U4C, Cell Signaling Technology, 1:1000), rabbit anti-phospho-mammalian target of rapamycin (mTOR Ser2448; D9C2, Cell Signaling Technology, 1:1000), rabbit anti-mTOR (7C10, Cell Signaling Technology, 1:1000), mouse anti-p53 (DO-1, sc-126, Santa Cruz Biotechnology, 1:500), mouse anti-pro-apoptotic effector B-cell lymphoma protein 2 (Bcl-2) Associated X (BAX; MA5-14003, Invitrogen, 1:500), rabbit anti-calnexin (ab22595, Abcam, 1:5000), mouse anti - β - actin (ab8224, Abcam, 1:5000), rabbit anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH; PA1-987, Invitrogen, 1:5000), rabbit anti-α-tubulin (ab6046, Abcam, 1:10000). .. Primary antibodies used for western blot analyses include mouse anti-EDEM3 (E0409, Sigma-Aldrich, 1:500), rabbit anti-EDEM1 (E8406, Sigma-Aldrich, 1:1000), rabbit anti-EDEM2 (JD-32) (sc - 130460, Santa Cruz Biotechnology, 1:250), rabbit anti-HBsAg (NB100-62652, Novus Biologicals, Littleton, CO, USA, 1:1000), rabbit anti-phospho-IRE1 alpha (Ser724) (PA1 - 16927, Invitrogen, 1:1000), rabbit anti-IRE1α (14C10, Cell Signaling Technology, Danvers, MA, USA, 1:1000), mouse anti - ATF6 (70B1413.1, Novus Biologicals, 1:1000), anti-glucose-regulated protein 94 (GRP94; H-10, Santa Cruz Biotechnology, 1:500), rabbit anti - phospho - PERK-T980 (16F8, Cell Signaling Technology, 1:1000), rabbit anti-PERK (C33E10, Cell Signaling Technology, 1:1000), rabbit anti - phospho-alpha subunit of eukaryotic initiation factor 2, S51(p - eIF2α; 9721S, Cell Signaling Technology, 1:1000), rabbit anti-eIF2α (9722S, Cell Signaling Technology, 1:1000), mouse anti-binding immunoglobulin protein (GRP78/BiP; A-10; sc-376768, Santa Cruz Biotechnology, 1:200), rabbit anti-suppressor of Lin - 12 - like (Sel1L; ab78298, Cell Signaling Technology, 1:500), rabbit anti-HMG-CoA reductase degradation protein 1, also known as Synoviolin (Hrd1; 14773, Cell Signaling Technology, 1:1000), rabbit anti-ER lectin 1 (XTP3-B/ERLEC1; ab181166, Abcam, Cambridge, UK, 1:500), rabbit anti-osteosarcoma amplified - 9 (OS-9; ab19853, Abcam, 1:500), rabbit anti-Ras homolog enriched in the brain (Rheb; E1G1R, Cell Signaling Technology, 1:1000), goat anti-apolipoprotein E (ApoE; AB947, Sigma-Aldrich, 1:5000), rabbit anti-microtubule-associated protein light chain 3A/B (LC3A/B; D3U4C, Cell Signaling Technology, 1:1000), rabbit anti-phospho-mammalian target of rapamycin (mTOR Ser2448; D9C2, Cell Signaling Technology, 1:1000), rabbit anti-mTOR (7C10, Cell Signaling Technology, 1:1000), mouse anti-p53 (DO-1, sc-126, Santa Cruz Biotechnology, 1:500), mouse anti-pro-apoptotic effector B-cell lymphoma protein 2 (Bcl-2) Associated X (BAX; MA5-14003, Invitrogen, 1:500), rabbit anti-calnexin (ab22595, Abcam, 1:5000), mouse anti - β - actin (ab8224, Abcam, 1:5000), rabbit anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH; PA1-987, Invitrogen, 1:5000), rabbit anti-α-tubulin (ab6046, Abcam, 1:10000).
Immunofluorescence:Article Title: Hepatitis B surface antigen is upregulated by HIV Tat in an HIV–hepatitis B virus co-infection model system
Article Snippet: .. Commercial primary antibodies used for Western blotting, immunofluorescence and flow cytometry were rabbit anti-HBV preS2 (PA5-22820; Invitrogen), rabbit anti-HBsAg (NB100-62652; Novas Biologicals, Littleton, CO), mouse anti-FLAG M2 (F1804; Sigma-Aldrich), rabbit anti-GFP (ab290; Abcam, Cambridge, UK), rabbit anti-GAPDH (2118; Cell Signaling Technology, Danvers, MA), rabbit anti-RNA polymerase II CTD, phospho S2 (ab193467; Abcam), and SureLight APC mouse anti-DDDDK tag (ab72569; Abcam). .. Anti-mouse IgG-HRP and anti-rabbit IgG-HRP or Alexa Fluor 488 and 568 (A11001, A11008, A11011, and A11031; Invitrogen) were used as secondary antibodies for Western blotting or immunofluorescence.
Flow Cytometry:Article Title: Hepatitis B surface antigen is upregulated by HIV Tat in an HIV–hepatitis B virus co-infection model system
Article Snippet: .. Commercial primary antibodies used for Western blotting, immunofluorescence and flow cytometry were rabbit anti-HBV preS2 (PA5-22820; Invitrogen), rabbit anti-HBsAg (NB100-62652; Novas Biologicals, Littleton, CO), mouse anti-FLAG M2 (F1804; Sigma-Aldrich), rabbit anti-GFP (ab290; Abcam, Cambridge, UK), rabbit anti-GAPDH (2118; Cell Signaling Technology, Danvers, MA), rabbit anti-RNA polymerase II CTD, phospho S2 (ab193467; Abcam), and SureLight APC mouse anti-DDDDK tag (ab72569; Abcam). .. Anti-mouse IgG-HRP and anti-rabbit IgG-HRP or Alexa Fluor 488 and 568 (A11001, A11008, A11011, and A11031; Invitrogen) were used as secondary antibodies for Western blotting or immunofluorescence.
Amplification:Article Title: The endoplasmic reticulum degradation-enhancing α-mannosidase-like protein 3 attenuates the unfolded protein response and has pro-survival and pro-viral roles in hepatoma cells and hepatocellular carcinoma patients
Article Snippet: Samples consisting of 50 tumor tissue (T), 50 normal adjacent to tumor tissue (NAT) and 10 normal (N) liver tissues were collected at the time of surgery in RNA stabilization solution (Sigma-Aldrich) and stored at −80 °C for further analysis. .. Primary antibodies used for western blot analyses include mouse anti-EDEM3 (E0409, Sigma-Aldrich, 1:500), rabbit anti-EDEM1 (E8406, Sigma-Aldrich, 1:1000), rabbit anti-EDEM2 (JD-32) (sc - 130460, Santa Cruz Biotechnology, 1:250), rabbit anti-HBsAg (NB100-62652, Novus Biologicals, Littleton, CO, USA, 1:1000), rabbit anti-phospho-IRE1 alpha (Ser724) (PA1 - 16927, Invitrogen, 1:1000), rabbit anti-IRE1α (14C10, Cell Signaling Technology, Danvers, MA, USA, 1:1000), mouse anti - ATF6 (70B1413.1, Novus Biologicals, 1:1000), anti-glucose-regulated protein 94 (GRP94; H-10, Santa Cruz Biotechnology, 1:500), rabbit anti - phospho - PERK-T980 (16F8, Cell Signaling Technology, 1:1000), rabbit anti-PERK (C33E10, Cell Signaling Technology, 1:1000), rabbit anti - phospho-alpha subunit of eukaryotic initiation factor 2, S51(p - eIF2α; 9721S, Cell Signaling Technology, 1:1000), rabbit anti-eIF2α (9722S, Cell Signaling Technology, 1:1000), mouse anti-binding immunoglobulin protein (GRP78/BiP; A-10; sc-376768, Santa Cruz Biotechnology, 1:200), rabbit anti-suppressor of Lin - 12 - like (Sel1L; ab78298, Cell Signaling Technology, 1:500), rabbit anti-HMG-CoA reductase degradation protein 1, also known as Synoviolin (Hrd1; 14773, Cell Signaling Technology, 1:1000), rabbit anti-ER lectin 1 (XTP3-B/ERLEC1; ab181166, Abcam, Cambridge, UK, 1:500), rabbit anti-osteosarcoma amplified - 9 (OS-9; ab19853, Abcam, 1:500), rabbit anti-Ras homolog enriched in the brain (Rheb; E1G1R, Cell Signaling Technology, 1:1000), goat anti-apolipoprotein E (ApoE; AB947, Sigma-Aldrich, 1:5000), rabbit anti-microtubule-associated protein light chain 3A/B (LC3A/B; D3U4C, Cell Signaling Technology, 1:1000), rabbit anti-phospho-mammalian target of rapamycin (mTOR Ser2448; D9C2, Cell Signaling Technology, 1:1000), rabbit anti-mTOR (7C10, Cell Signaling Technology, 1:1000), mouse anti-p53 (DO-1, sc-126, Santa Cruz Biotechnology, 1:500), mouse anti-pro-apoptotic effector B-cell lymphoma protein 2 (Bcl-2) Associated X (BAX; MA5-14003, Invitrogen, 1:500), rabbit anti-calnexin (ab22595, Abcam, 1:5000), mouse anti - β - actin (ab8224, Abcam, 1:5000), rabbit anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH; PA1-987, Invitrogen, 1:5000), rabbit anti-α-tubulin (ab6046, Abcam, 1:10000). .. Primary antibodies used for western blot analyses include mouse anti-EDEM3 (E0409, Sigma-Aldrich, 1:500), rabbit anti-EDEM1 (E8406, Sigma-Aldrich, 1:1000), rabbit anti-EDEM2 (JD-32) (sc - 130460, Santa Cruz Biotechnology, 1:250), rabbit anti-HBsAg (NB100-62652, Novus Biologicals, Littleton, CO, USA, 1:1000), rabbit anti-phospho-IRE1 alpha (Ser724) (PA1 - 16927, Invitrogen, 1:1000), rabbit anti-IRE1α (14C10, Cell Signaling Technology, Danvers, MA, USA, 1:1000), mouse anti - ATF6 (70B1413.1, Novus Biologicals, 1:1000), anti-glucose-regulated protein 94 (GRP94; H-10, Santa Cruz Biotechnology, 1:500), rabbit anti - phospho - PERK-T980 (16F8, Cell Signaling Technology, 1:1000), rabbit anti-PERK (C33E10, Cell Signaling Technology, 1:1000), rabbit anti - phospho-alpha subunit of eukaryotic initiation factor 2, S51(p - eIF2α; 9721S, Cell Signaling Technology, 1:1000), rabbit anti-eIF2α (9722S, Cell Signaling Technology, 1:1000), mouse anti-binding immunoglobulin protein (GRP78/BiP; A-10; sc-376768, Santa Cruz Biotechnology, 1:200), rabbit anti-suppressor of Lin - 12 - like (Sel1L; ab78298, Cell Signaling Technology, 1:500), rabbit anti-HMG-CoA reductase degradation protein 1, also known as Synoviolin (Hrd1; 14773, Cell Signaling Technology, 1:1000), rabbit anti-ER lectin 1 (XTP3-B/ERLEC1; ab181166, Abcam, Cambridge, UK, 1:500), rabbit anti-osteosarcoma amplified - 9 (OS-9; ab19853, Abcam, 1:500), rabbit anti-Ras homolog enriched in the brain (Rheb; E1G1R, Cell Signaling Technology, 1:1000), goat anti-apolipoprotein E (ApoE; AB947, Sigma-Aldrich, 1:5000), rabbit anti-microtubule-associated protein light chain 3A/B (LC3A/B; D3U4C, Cell Signaling Technology, 1:1000), rabbit anti-phospho-mammalian target of rapamycin (mTOR Ser2448; D9C2, Cell Signaling Technology, 1:1000), rabbit anti-mTOR (7C10, Cell Signaling Technology, 1:1000), mouse anti-p53 (DO-1, sc-126, Santa Cruz Biotechnology, 1:500), mouse anti-pro-apoptotic effector B-cell lymphoma protein 2 (Bcl-2) Associated X (BAX; MA5-14003, Invitrogen, 1:500), rabbit anti-calnexin (ab22595, Abcam, 1:5000), mouse anti - β - actin (ab8224, Abcam, 1:5000), rabbit anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH; PA1-987, Invitrogen, 1:5000), rabbit anti-α-tubulin (ab6046, Abcam, 1:10000).
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